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��-Carotene bleaching assay In this assay, ��-carotene is oxidized by free radicals derived from the oxidation of linoleic acid, which can better simulate the food system compared to DPPH assay (Razali et?al. 2012). The auto-oxidation products of linoleic acid attack the double bonds of ��-carotene and in the absence oxyclozanide of an antioxidant the ��-carotene molecule loses its chromosphere and undergoes rapid discoloration which can be monitored spectrophotometrically (Martinez-Correa et?al. 2011). However, in the presence of an antioxidant, ��-carotene retains its original yellow color by scavenging the free radicals formed in the system (Razali et?al. 2012). Effect of loquat fruit skin and pulp extracts on oxidation of ��-carotene/linoleic acid emulsion is shown in Table?Table3.3. It was clear that the presence of extracts reduced the oxidation of ��-carotene. There were significant differences (P?this website and SSW in level 1000?ppm were higher than other samples. Overall, the antioxidant activity effect of skin extracts on reducing the oxidation of ��-carotene was higher than the control and pulp extracts, but the best antioxidant power was observed in TBHQ. This suggests that the content of phenolic and tocopherol compounds can play a major role in the antioxidant activity of skin extracts compared to pulp extracts. Results also showed that solvent extraction was more effective on antioxidant activity of loquat extracts compared to other methods. These results concurred with Koba et?al. (2007) which examined the antioxidant effect of loquat fruit skin and pulp by the ��-carotene bleaching assay. Table 3 Antioxidant activity of loquat fruit skin and pulp extracts in a ��-carotene/linoleate model system. Rancimat analysis The Rancimat analysis was performed Decitabine clinical trial at 120��C and the induction period (h) was evaluated for soybean oils with or without loquat fruit skin and pulp extracts at 400 and 1000?ppm. Our results showed both skin and pulp extracts had a strong antioxidant activity in soybean oil. As it has been shown in Table?Table4,4, the presence of extracts retarded the oxidation of soybean oil, so that the lowest thermal stability in oils treated with extracts was for the EPC sample (3.8?h), which was higher compared to the control oil (3.32?h). On the other hand, the highest thermal stability in oils containing loquat fruit skin and pulp extracts was for SSEW at 400 and 1000?ppm (4.69 and 4.49?h), but the best protection effect was observed in oil containing 100?ppm of TBHQ. Therefore, results also showed that the skin extracts was more effective in prevention of soybean oil oxidation compared to pulp extracts.