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The interassay variabilities of plasma GPx and GR activities were 4.4% (n=3) and 6.8% (n=3), respectively, and the intraassay variabilities GPx and GR activities were 6.1% (n=5) and 6.2% (n=5), respectively. Protein content was determined using the method of Lowry et al. (24). Results were expressed in nmol/mg selleck chemicals llc protein for GSH and nmol/min/mg protein for GPx and GR activity levels. All analyses were performed in duplicate. Statistical analyses Data were analyzed using the SAS statistical software (version 9.3; Statistical Analysis System Institute Inc., Cary, NC, USA). A Kolmogorov�CSmirnov test was performed to determine the normal distribution. Biochemical values are compared for significant differences using one way analysis of variance among groups. Because some data were skewed rather than normally distributed, differences among groups were then determined using the Kruskal�CWallis one way analysis of variance on ranks. Student�CNewman�CKeuls test was used for the post-hoc analysis. Correlations of PLP with cysteine, GSH, and antioxidant enzyme activities were determined using the Pearson correlation coefficient. Results were considered statistically significant at pQuinapyramine concentrations in plasma and liver. Mice fed the vitamin B6-deficient diet had significantly decreased plasma PLP concentrations among groups. On the contrary, plasma PLP significantly increased when the vitamin B6-deficient diet was supplemented with 14 mg/kg of vitamin B6. Although control and homocysteine groups had adequate vitamin B6 intake, plasma and liver PLP concentration significantly decreased in the homocysteine group with homocysteine added to the drinking water. Homocysteine-treated mice had significantly higher homocysteine concentration in plasma and liver when compared with mice not given homocysteine. Among the homocysteine-treated groups, mice with the vitamin B6-deficient diet had the highest homocysteine concentrations in plasma see more and liver when compared with those of mice with normal diet or vitamin B6-supplemented diet. Plasma cysteine concentrations were significantly reduced in the vitamin B6-deficient group when compared with other groups, but there were no significant changes in cysteine concentrations in liver. Table 2 Vitamin B6, homocysteine, and cysteine in plasma and liver Concentrations of the oxidative stress indicator, GSH, and its related enzyme activities are listed in Table 3. There were no significant changes in plasma MDA concentration among groups. Significantly increased hepatic MDA levels were only observed in the vitamin B6-deficient group. Among homocysteine-treated groups, mice with vitamin B6-deficient diet had the highest plasma GSH concentration and relatively lower hepatic GSH concentration.