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(A) The expression of TMEM16A protein was detected by Western blotting in dissociated normal sinonasal epithelium (control group) and nasal polyp epithelium (HNPE group). ... Location of Selleck JNJ 26481585 TMEM16A and MUC5AC in ALI-cultured HNPECs To further investigate the localization of TMEM16A and MUC5AC further, we performed immunofluorescence combined with confocal microscopy to assess the expressions of TMEM16A, MUC5AC, and acetylated-tubulin protein (a marker for ciliated cells) in ALI-cultured HNPECs incubated with or without IL-13 (10 ng/mL) for 14 days. TMEM16A expression was mainly expressed in MUC5AC-positive cells in cultured HNPECs incubated with IL-13, with a weak expression noted in the control group. MUC5AC protein was localized in mucus granules, and TMEM16A protein was localized on the circumference of the MUC5AC protein (Fig. 3A and B). TMEM16A and acetylated-tubulin proteins were not coexpressed within the same cells in HNPECs incubated with or without IL-13, indicating that TMEM16A was not expressed on ciliated cells (Fig. 4A and B). Fig. 3 Coexpression (-)-p-Bromotetramisole Oxalate of TMEM16A and MUC5AC in ALI-cultured HNPECs. Confocal microscopy xy images of ALI-cultured HNPECs without IL-13 (A) and with IL-13 treatment (10 ng/mL, 14 days) (B) showing over lapping immunoreactivity for TMEM16A (red) and MUC5AC (green). ... Fig. 4 Expression of TMEM16A in non-ciliated cells. Confocal microscopy xy images of ALI-cultured HNPECs without IL-13 (A) and with IL-13 (10 ng/mL, 14 days) (B) showing immunoreactivity for TMEM16A (red) and acetylated-tubulin (green). Nuclei stained with DAPI ... Effect of IL-13 on the expression of TMEM16A and the secretion of MUC5AC in ALI-cultured HNPECs The percentage selleck chemicals llc of TMEM16A-positive cells and MUC5AC-positive cells was significantly higher in HNPECs incubated with IL-13 10 ng/mL for 14 days (23.0%��2.5% and 21.9%��6.0% of total cells, respectively) than in those which was not (4.4%��0.7% and 3.9%��1.5% of total cells, respectively; both P