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The sections were mounted onto glass slides and stained with FGF-2 antibody, which was performed at Kyodo Byori Inc. (Hyogo, Japan). All animal experiments were approved and carried out following the approval and guidelines for animal experimentation set by the National Defense Medical College, Saitama, Japan. Participants of clinical research The participants were 12 individuals (eleven men, one woman) with ages ranging from 41 years to 77 years (mean age, 58.3 years) with thin hair and without any treatment of thin hair for at least 6 months. The participants visited the hospital monthly to receive inspections and bottles of FGF-2&D/P NPs. Participants were photographed using a digital camera for general observation and a hair diagnosis system (Folliscope; LeadM Corp, Seoul, South Korea) for measuring hair diameter. All research protocols were submitted and approved by the selleckchem ethics committee of the National Defense Medical College (clinical trial No. 1148 and No. 1241) and registered in the University Hospital Medical Information Network Center (UMIN000011748). Each participant was informed of the protocols and signed a written consent form before participating in the study. The application and evaluation of FGF-2&D/P NPs on the skin of the scalp One milliliter of FGF-2&D/P NPs was applied and massaged on the skin of the scalp by participants twice a day. Monthly observations were conducted on the participants for 6 months. The Folliscope captured ten dermoscopic images at 1 cm intervals toward the front left from the parietal region. The diameters of all hair shafts in each dermoscopic image were measured. CASK The check details mean diameter of all hairs and the percentage of terminal hair (with diameter >0.040 mm) were calculated using the measured data. All values are expressed as mean �� SD. Statistical analysis was performed with Wilcoxon signed-rank test using JMP Pro 11.2.0 software (SAS Institute Inc., Cary, NC, USA). P