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After ultra-thin sectioning, cells were examined using a transmission electron microscope at a magnification of 1,500X. Flow cytometry analysis Cell death was detected using the MUSE? Annexin-V Dead Cell kit (EMD Millipore Corporation, Hayward, CA, USA). In our experiments, SKOV3 and SKVO3/DDP cells were seeded in 6-well culture plates at a density of 2��105 cells/well. After treatment under different experimental conditions, cells were trypsinized and resuspended in RPMI-1640 medium with 10% FBS at a concentration of 1��106 cells/mL. Under darkroom conditions, cells were incubated with Annexin-V at room temperature for 20 min. Finally, cells were measured using the MUSE? Cell Analyzer (EMD Millipore Corporation, Hayward, CA, USA). All experiments were performed AG-014699 nmr in triplicate. Ovarian cancer tumor xenograft in female nude mice Athymic BALB/c female nude mice (4 weeks old, weight 20g) were purchased from Vital River Laboratory Animal Technology Co. Ltd, Beijing, China. Female nude mice were maintained in specific pathogen-free conditions and environment under controlled conditions of 12-h light/12-h dark cycle for three days. A xenograft model of human ovarian cancer in female nude mice was established through subcutaneously injecting SKOV3 or SKOV3/DDP cells into the right of each mouse. The mice were randomly divided into 4 groups, which were the control group of SKOV3 cells, the cisplatin group of SKOV3 cells, the control group of SKOV3/DDP cells, and the cisplatin group of SKOV3/DDP cells. Treatment was Quetiapine started when the tumour reached about 150 mm3-200mm3. 2mg/ml cisplatin was intraperitoneally injected once two days. Tumor diameter was measured using a caliper every 2 days, and the tumor volume was calculated according to the formula: length �� width �� height �� 0.5. After treatment with cisplatin, the tumors were excised. Statistical Analysis Data are representative of three independent experiments performed in triplicate LDK378 and presented as the means �� SD. Data analysis was performed using one-way analysis of variance. Tukey��s post-hoc test was used to determine statistical significance of all pairwise comparisons of interest; p