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Alternatively, four-day-old MoM?s were treated with either IL-10 (D), or with dexa (E). Light microscopy photographs were taken after 24 h with GDC-0199 purchase cytokines and are representative of three independent experiments. Magnification is 20��. Click here for additional data file.(182K, JPEG) Click here for additional data file.(32K, JPEG) FIGURE S2 | Data summarizing flow cytometric analysis of M1 and M2 MoM? endocytosis. The ability of unstimulated MoM?s or MoM?s treated with M1 or M2 cytokines to endocytose was assessed using APC*-labeled OVA. Cells were incubated with OVA at 4��C as a negative control (blue dots), or at 37��C (red dots), for 1 h before flow cytometric analysis of APC* fluorescence. Dots represent individual experiments, showing the percentage of cells fluorescing APC*, indicating endocytic OVA uptake. Lines represent mean percentage of cells associated with OVA-APC* ��SEM. Click here for additional data file.(32K, JPEG) Click here for additional data file.(32K, JPEG) FIGURE S3 | Data summarizing flow cytometric analysis of M1 and M2 MoM? phagocytosis. The ability of unstimulated MoM?s or MoM?s treated with M1 or M2 cytokines to phagocytose was assessed using FITC-labeled microsphere particles. Cells were incubated with particles Casein kinase 2 at 4��C as a negative control (blue dots), or at 37��C (red dots), for 3 h before flow cytometric analysis of FITC fluorescence. Dots represent individual experiments, showing the percentage of cells fluorescing FITC, indicating phagocytic uptake. Lines represent mean percentage of cells associated with FITC microsphere particles ��SEM. Click here for additional data file.(32K, JPEG) Click here for additional data file.(34K, JPEG) FIGURE S4 | Endocytic Activity Raf tumor of MoM?s following dexa and IL-10 treatment. The ability of unstimulated MoM?s or MoM?s treated with dexa or IL-10 to endocytose was assessed using APC*-labeled OVA. Cells were incubated with OVA for 1 h at 4��C (blue) or 37��C (red) before flow cytometric analysis of percentages of cells associated with APC*-OVA. Dots represent individual experiments; lines represent mean percentages ��SEM. One-way ANOVA was used to assess significance followed by Bonferroni��s multiple comparison test, *p