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0, RRID: nlx_155747 (PST Software Inc., Pittsburgh, PA). Data analysis Questionnaire and demographic data were analyzed using Excel 2007 (Microsoft, Redmond, PA) and SPSS (http://www-01.ibm.com/software/analytics/spss/), including assessment on their distribution and mean scores, and values were centered (Z-scores) to avoid FMO4 collinearity issues (Aiken and West 1991). Relations between behavioral data and questionnaire measures as well as age were computed using multiple regression analyses, by entering performance as dependent, and attachment questionnaire scores as well as age and sex as independent variables. A separate independent samples t-test analysis was computed regarding sex differences by controlling for attachment Akt inhibitor avoidance, anxiety, and age. Functional images were analyzed using SPM Version 8, Revision Number 4290, RRID: nif-0000-00343 (Wellcome Department of Imaging Neuroscience, London, UK; http://www.fil.ion.ucl.ac.uk/spm) under Matlab. EPI volumes were realigned, normalized to the MNI (Montreal Neurological Institute) template, resampled to 2 mm3, and spatially smoothed using a 8-mm FWHM Gaussian kernel. Coordinates thus refer to millimeters in the MNI stereotaxic space. For each participant, the six different conditions (SFW, SFL, AFW, AFL, as well as neural activity during dot perception, either on ��Won�� [DW] or ��Lost�� [DL] trials��used as a baseline) were modeled as single events and convolved with the standard hemodynamic response (Vrticka et al. 2008). The first-level model also included four additional conditions (SFW-M, SFL-M, AFW-M, and AFL-M) representing brain activity during a subsequent memory task, because this memory task was scanned immediately after the session of interest for the present investigation. However, these additional memory conditions will not be considered here. Realignment parameters were incorporated as six additional regressors of no interest. During the estimation of the model, a high-pass frequency filter (cutoff 128 s) and corrections Sotrastaurin concentration for autocorrelation between scans were applied to the time series. Random effects were evaluated by combining contrast images computed from individual analyses. First, we computed the main effects contrast of objective performance feedback ([SFW + AFW] vs. [SFL + AFL]), facial emotional expressions ([SFW + SFL] vs. [AFW + AFL]), and their interaction (congruent [SFW + AFL] vs. incongruent [SFL + AFW] social evaluation), and further decomposed these contrasts by computing comparisons between two experimental conditions only (e.g., SFW vs. AFW, etc.). These analyses were carried out at P