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4, 5D, right), cytoplasmic staining showed a pattern similar to that of the 1 �� 2-min tCCAO group, but with the peak time accelerated to 7 days. Among total see more FUS/TLS-positive cells, the ratios of cytoplasmic positive pyramidal neurons were 14.0%?��?7.4%, 23.0%?��?9.1%, 36.1%?��?13.3%, 26.8%?��?12.1%, 25.8%?��?9.3%, and 21.1%?��?8.8% at 1, 3, and 7 days (���P?GUCY1B3 cells/mm2, **P?BKM120 chemical structure CA1 in hippocampus (Fig. 2, right), as in a previous study (Kato et al., 1989). However, the three times mild ischemia group exhibited a significant recovery of the CA1 pyramidal neurons after 7 days to 6 months (Fig. 2B, right). In the present study, significant changes in TDP43 and FUS/TLS were shown in the ischemic brains (Figs. 3-5). Upregulation of TDP43 expression with prominent cytosolic localization was found in motor neurons of ALS, which may represent an appropriate response to neuronal injury (Moisse et al., 2009).