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1). Fig. 1 Typical representation of equol-induced response of human uterine arterial strip on precontracted status by phenylephrine (10-5 M). Cumulative application of equol evoked dose-dependent relaxation. PE, phenylephrine. (2) The effects of equol on inducement of vasospasm by a high concentration potassium solution After the contraction of the uterine arterial smooth muscle by a high concentration potassium chloride solution 70 mM in the same way, when administered equol in the same concentration range (from 10-11 to 10-6 M), the contractile response of smooth muscle were not shown with any significant relaxation response (Fig. 2). Fig. 2 Typical representation of equol-induced response of human uterine arterial strip see more on precontracted status by high concentration of KCl (70 mM). Cumulative application of equol evoked no remarkable change. (3) Evaluation of vasospasm with phenylephrine and a high concentration of potassium solution after selleck chemicals llc pretreatment of equol After pretreatment of uterine arterial smooth muscle using equol 10-8 M first, and after induction of vasospasm using the six concentrations of phenylephrine RRAD from 10-9 to 10-4 M and the four of highly concentrated potassium solutions from 35 to 140 mM, the effects of pre-treated equol was evaluated. The vasospasm induced by the six different concentrations of phenylephrine from 10-7 M had shown concentration-dependent significant reduction in the group of pretreatment with equol (Fig. 3, Table 1), whereas vasocontraction by the other six concentrations of phenylephrine from 10-7 M showed a concentration-dependent significant reductions in the pretreated equol groups (Fig. 3, Table 1) but the vasospasm induced by the four of highly concentrated potassium solution did not have significant differences regardless of whether the pretreatment of equol at all concentrations of potassium solution (Fig. 4, Table 2). Fig. 3 Effects of 10-8 M equol-pretreatment on the phenylephrine-induced contractures. The responses were decreased and the effects were statistically significant (n=8, *means P