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Data analysis Frequency domain measures of ECG R-R variability were used to estimate the high frequency (HF, 0.15�C0.4 Hz) and low frequency (LF, 0.04�C0.15 Hz) power. The HF power peak represents modulation of efferent parasympathetic (vagal) activity by ventilation, but Alizarin only in the presence of sinus rhythm. The LF power peak represents combined modulation of efferent parasympathetic and efferent sympathetic nervous system activity by baroreflex activity. Overall the amplitudes of LF and HF power reflect modulation of sinus node firing rate and the LF/HF ratio (HRV ratio) has been proposed as an index of sympathovagal balance.9,13 However, very LF indices could not be used since they require power spectra that are more than 5 minutes long, which was not in keeping with our experimental protocol. The IWORX Labscribe 2 software which is part of the physiological system was used to analyze these variables (iWorx Sytems, Inc.). SPSS software version 16.0 (SPSS Inc., Chicago, IL, USA) was used to analyze the data. Independent samples two-tailed Student��s t-test at 5% level of significance was used to compare the various means of the cardiovascular parameters between NBW and LBW individuals (PSB203580 clinical trial and as mean and standard deviation (SD) in tables. Results A total of 23 LBW (18 females and five males) and 23 NBW (14 females and nine males) black adults, with a median age of 20 years (18�C25 years), successfully completed the experiment. The baseline characteristics and mean MVC, time to fatigue as well as the heart Fulvestrant chemical structure rate changes are shown in Tables 1 and ?and2,2, respectively. Table 1 Baseline demographic characteristics of the study sample Table 2 Mean heart rates during entire protocol and mean maximum voluntary contraction and time to fatigue HRV ratio The trends of mean HRV ratios for LBW and NBW individuals during the experimental protocol are shown in Figure 1. The mean increase in HRV from baseline to exercise was greater for LBW compared to NBW individuals (1.015��1.034 versus [vs] 0.119��0.789, respectively; P