The Secrets On GSK2118436 Uncovered In Seven Days Easy Steps

In this issue of Cell Host & Microbe, Caradonna et?al. describe a particularly sophisticated set of studies founded upon an unbiased, genome-scale, forward genetic screen to identify host genes and proteins, as well as their?participatory metabolic partners, that impact the intracellular growth of T.?cruzi. The primary screen conducted in HeLa cells consisted of a genome-wide knockdown of 18,263 host mRNAs and their corresponding proteins using siRNA technology and offers a powerful, all-encompassing approach for the identification of both positive and negative regulators of T.?cruzi growth. The significance of the data from the primary screen, which yielded hundreds of GSK2118436 mw hits?(see Table S1 in Caradonna et?al., 2013), was realized by the implementation?of?an?ingenious secondary screen PD-0332991 mouse to distinguish those host proteins important for T.?cruzi early infection��i.e., trypomastigote invasion and early establishment in the host cell (Megestrol Acetate of very long chain fatty acids by the host cell and their subsequent oxidation is likely a requisite for parasite viability and ultimately, perhaps, for parasite persistence. A key question for future studies will be how the parasite manipulates and ultimately benefits from these changes in the host metabolic landscape. Whether this is a ploy to mobilize and obtain fatty acid precursors necessary for the synthesis of membrane in the dividing amastigote population or, alternatively, to bolster parasite energy production by?providing reducing intermediates (i.e., NADH and FADH2) generated during the?oxidation process remains to be elucidated.