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, 2000). At early endosomes, the kinesin-3 KIF16B regulates the motility of SEs (Hoepfner et?al., 2005) or common REs in epithelial cells (Perez Bay et?al., 2013), and the kinesin-2 KIF3B has been implicated in the endosomal sorting of Tf (Schonteich et?al., 2008). Although RE tubules align along microtubules near SEs (Willingham et?al., 1984), the KIF required for generation of RE tubules from the vacuolar SEs has not been defined. The plus-end-directed kinesin-3, KIF13A, is Caspase inhibitor a good candidate to fulfill such a function. In pigment cells, KIF13A coordinates the positioning of RAB11-positive endosomes in the cell periphery with protein sorting to nearby melanosomes (Delevoye et?al., 2009). Here, we demonstrate that KIF13A dynamically associates with REs and is essential for the generation of RE tubules from SEs and for their distribution toward the cell periphery. Moreover, KIF13A binds to the active GTP-bound form of the RE-associated RAB11 to control endosomal sorting and recycling of an endosomal cargo. We first investigated KIF13A localization in HeLa cells by immunofluorescence microscopy (IFM). A KIF13A-YFP fusion protein localized to clustered puncta at the cell periphery that codistributed partially with classical RE components, as shown by Mander��s coefficient (MC) analysis of overlap with internalized Tf (0.43 �� 0.1; p?Ceftiofur p?Ceritinib TGN46 distribution (Figure?S1F). These observations support our previous studies showing that endogenous KIF13A localized partially with RE markers in melanocytes (Delevoye et?al., 2009) and show that the bulk of KIF13A associates with REs. Expression of KIF13A-YFP (Figures 1A and S1A, arrows; Movie S1), but not of GFP alone (Figure?S2A), caused a pool of Tf/TfR-positive endosomes to accumulate at the cell periphery. Moreover, overexpression of KIF13A, but not GFP, induced dramatic tubulation of membranes that labeled for endogenous RAB11A and RCP (Figures 1B and S1B, arrows; Figure?S2B; Movie S2). KIF13A-positive tubular membranes aligned along microtubules (Figure?S2C, arrows).