Two Oxymatrine Cons And Ways To Block It

Restriction digestion of purified PCR products was conducted using 10 ��l of purified DNA, 5-20 U restriction enzyme, and 1x corresponding restriction enzyme buffer, made up to a total volume of 20 ��l with nuclease-free water. Following restriction digestion, the samples were separated using agarose gel electrophoresis (1-5%, depending on product size), labeled with SybrSafe (Invitrogen) and visualized under ultraviolet (UV) light. Genotype calling was conducted blindly by two independent reviewers. RESULTS Hardy�CWeinberg equilibrium and genotype frequencies for PD survivors www.selleckchem.com/products/verubecestat-mk-8931.html and control group participants were compared using Fisher's exact test. Analyses were performed using IBM Statistical Package for Social Sciences (SPSS) 21. Oxymatrine Genotype frequencies were considered to be different between groups at P find more in the MTHFR gene (��2 = 5.68, P