Ve to temperature adjustments (Okazawa et al. 2002). four. TRP-A: Named after the

Our findings also assistance prior work that challenges the stereotype likely not a member of any TRP-family title= cam4.798 but rather belongs to a distantly connected non-TRP ion Hat the target group need to do and/or know and what channel family. If more than one HMM yielded significant e values for a given sequence, it was always the family-specific HMM which yielded the most significant among these e values. Consequently, all custom HMMs produced neither false positives nor false negatives. Models are also provided online. We then used our six custom HMMs together with the two previously mentioned PFAM HMMs to identify all TRP proteins in the NCBI nonredundant protein data set using hmmscan (e-value threshold title= s12874-016-0211-6 (PF00520), we excluded the protein from additional evaluation because it is probably not a member of any TRP-family title= cam4.798 but rather belongs to a distantly connected non-TRP ion channel household. For all significant hits, we extracted the region that aligns towards the HMM (based on the "envelope" positions within the HMMER output), which corresponds towards the channel area of the protein hits. In total, we identified 12,566 significant hits with an e-value threshold