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A total number of 945 and 746 different proteins from body and headfoot samples respectively were identified over all time points studied (confidence threshold >?95%); of these, 452 proteins were common to both samples (Fig.?1A). Of all the proteins identified, only those whose credible interval (from iQuantitator analysis) was above or below 1 and whose log2 fold-change was >?0.6 or flupentixol investigation. A total of 108 significantly differentially expressed proteins were found in the body samples, whereas only 43 proteins were differentially expressed in the headfoot of the infected snails (Fig.?1B). A comprehensive report was also generated with the iQuantitator software (Supplementary Files 1�C4 in [37]). A GO-enrichment analysis of significantly differentially expressed proteins from the body and the headfoot of infected snails was performed using Blast2GO [35]. The analysis revealed significant enrichment OSI-906 cost of the GO terms ��binding�� (13.2% and 14.4% in body and headfoot, respectively), ��catalytic activity�� (11.4% and 12.3%) and ��protein binding�� (8.3% and 9.8%) within ��molecular function�� (Fig.?2A) and ��single-organism cellular process�� (9.3% and 8%), ��regulation of biological process�� (8.7% and 8%), ��primary metabolic process�� (8.1% and 9.3%) and ��organic substance metabolic process�� (8.1% and 9.3%) within ��biological process�� (Fig.?2B). No significant differences were observed between enriched GO terms from Lapatinib molecular weight the body and headfoot of infected snails. Significantly differentially expressed proteins from the headfoot of infected snails were grouped into 8 GO annotation categories and plotted in a clustered heatmap (Fig.?3). Clustering was performed using Euclidean distances and dendrograms were reordered based on mean values. Proteins assigned to peptidase activity, and oxidoreductases (with the exception of 15-hydroxyprostaglandin dehydrogenase) together with proteins with a catalytic domain were significantly downregulated after infection with O. viverrini. Conversely, proteins involved in motor activity and structural proteins were upregulated in the headfoot of infected snails among the experiment ( Fig.?3). Significantly dysregulated proteins from the bodies of infected snails were grouped into 10 GO annotation categories and plotted in a clustered heatmap (Fig.?4). Clustering was also performed using Euclidean distances and dendrograms were reordered based on mean values. The majority of differentially expressed proteins were identified at 28�C56?dpi, and similar numbers of up- and downregulated proteins were detected.