Vital Aspects Why You Should Never Doubt The Capacity Of LEE011

Calcd. for C20H30N2O3: C, 69.35; H, 8.67; N, 8.05. Found: C, 69.33; H, 8.73; N, 8.09. t-butyl 2-[4-(N-phenylpropionamido)piperidin-1-yl]propionate (8) Colorless solid (74%); mp 98�C100��C. IR (KBr) ��max 3330, 3030, 2984, 2880, 1740, 1652, 1470, 1113, 1006, 929, 801; 1H NMR [CDCl3, 400 MHz): ��=1.00 (t, 3H, H-20), 1.18 (d, 3H, H-8), 1.34 (m, 2H, H-3ax, 5ax), 1.45 (s, 9H, H-13, 14, 15), 1.75 (m, 2H, H-3eq, 5eq), 1.90 (q, 2H, H-19), 2.35�C2.47 (m, 2H, H- H-2ax, 6ax), 2.90 (bd, 2H, H-2eq, 6eq), 3.10 (q, 1H, H-7), http://www.selleckchem.com/products/lee011.html 4.66 (m, 1H, H-4), 7.07 (d, 2��Ar-H), 7.38 (m, 3��Ar-H); 13C NMR [CDCl3, 100 MHz): ��=9.6 (CH3, C-20), 15.1 (CH3, C-8), 28.3 Resiquimod (CH2, C-19), 28.5 (CH3, C-13, 14, 15), 31.0 (CH2, C-3, 5), 47.4 (CH2, C-2, 6), 50.9 (CH, C-4), 52.4 (CH, C-7), 63.1 (C, C-12), 128.2 (CH, C-22, 26), 129.3 (CH, C-24), 130.5 (CH, C-23, 25), 138.9 (C, C-21), 172.4 (CO, C-17), 173.5 (CO, C-9); EI-MS m/z (pos): 360, 303, 289, 277, 259, 216, 203, 187, 156, 146, 132, 110, 93, 82, 56; Anal. Calcd. for C21H32N2O3: C, 70.02; H, 8.05; N, 7.58. Found: C, 69.97; H, 8.95; N, 7.77. Biological assay Animals Male Swiss albino mice (25�C30 g) were procured from the Animal Facility of the Defence Research and Development Establishment (DRDE), Gwalior. The animals were housed in polypropylene cages on dust free rice husk as bedding material, with free access to food (Ashirwad Brand, Chandigarh, India) and water ad libitum. Prior to experiment, the animals were randomized and acclimatized for seven days in controlled environmental conditions (22��2��C; relative humidity 40�C60%) at a 12h light/12 h dark cycle. The care and maintenance of the animals were as per the approved guidelines of the Committee for the Purpose of Control and Supervision of Experiments selleck screening library on Animals (CPCSEA), Ministry of Environment and Forest, Govt. of India, New Delhi, India. The experimental protocol was approved by the Institutional Ethical Committee on Animal Experimentations approved by CPCSEA. Determination of LD50 The LD50 of the compounds was determined by intravenous (i.v.), intraperitoneal (i.p.) and oral (p.o.) routes following Dixon's up and down method (Dixon, 1965), using 4�C6 mice for each value. All the compounds were dissolved in DMSO and administered in a volume