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Normal distribution was tested using a D��Agostino and Pearson omnibus normality test and homogeneity of variances was tested using a Levene��s test. Single and multi-level regression analyses were used to investigate changes in performance. A hierarchical regression model was used to avoid the impact of a cluster-effect on results in case one athlete finished more than once in the annual top or annual top ten. Since the change in sex difference in endurance performance is assumed to be non-linear [11], we additionally calculated the non-linear regression model that fits the data best. The result of the linear regression analysis was compared to the best-fit result of the non-linear analysis using Akaike��s Information Criteria (AIC) and F-test in order to show which model would be the most appropriate to explain the trend of the data. Split times in swimming, Sitaxentan cycling, and running as well as overall race times and transition times in top ten women and men and differences in transition times between ��Ironman Hawaii�� and ��Ironman 70.3�� in top ten men and women were compared using multiple t-tests CAL 101 with Holm-Sidak correction for multiple comparisons. Statistical analyses were performed using IBM SPSS Statistics (Version 22, IBM SPSS, Chicago, IL, USA) and GraphPad Prism (Version 6.01, GraphPad Software, La Jolla, CA, USA). Significance was accepted at P?Afatinib cost the transition times remained unchanged across years for the annual fastest triathletes, but decreased linearly for the annual ten fastest triathletes from 48.0?�� 47.6% (1998) to 18.9?�� 28.3% (2013). Figure 1 Changes in transition times and sex difference in transition times in ��Ironman Hawaii�� for the annual fastest and the annual ten fastest in absolute (i.e. min) and relative terms (i.e. expressed in % of overall race times) from 1998-2013. ... Table 1 Multi-level regression analyses for change in transition times ( i.e. in absolute times and relative in % of overall race times) for the annual fastest and annual ten fastest across years after correction for multiple finishes in ��Ironman Hawaii�� ... Changes in transition times in ��Ironman 70.3�� In ��Ironman 70.3�� (Figure?2), the transition times remained unchanged for the annual fastest in both absolute and relative terms (Table?2).