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At shorter distances to the root tip ( tip. In the sections taken from 6 to 9 mm from the tip, the epidermis, exodermis, and protoxylem were detected at both ploidy levels, but the FKBPL developmental stage was more advanced in the 4x root sections than in the 2x ones. At 9�C12 mm from the apex, the Casparian strip, located on the endodermis cell layer, was clearly identifiable as dots, which surrounded the vascular cylinder in the 4x roots (Figure ?Figure4B4B), while it was only slightly detected in the 2x roots (Figure ?Figure4A4A). At a distance of 12�C15 mm from the tip, the Casparian strip and the vascular cylinder appeared in an advanced stage of development. Thus the tissue differentiation in the 4x roots was presumed to take place in a shorter distance from the root tip in 4x than in 2x. Moreover, the root tip exodermis was broader in the 4x genotype (Figure ?Figure4B4B) than in 2x (Figure ?Figure4A4A), and the suberin deposition in the cell walls also appeared thicker in the former. FIGURE 4 Free-hand cross-sections of the roots of (A) the 2x and (B) 4x CC seedlings Trametinib molecular weight grown in vitro, taken at 9�C12 mm from the root apex, cleared in chloral hydrate, stained with berberine Olaparib hemisulfate, post-stained with aniline blue and visualized under ... Boron Excess Effect on 2x and 4x Plants Previous Description of Plant Morphology At the beginning of the experiment, the 6-month-old 2x and 4x seedlings had similar DW (Table ?Table55) and shoot architecture, since height and leaf number did not differed between them (data not shown). However, the root system morphology was significantly influenced by ploidy. The 4x roots appeared thicker, shorter and had fewer secondary rootlets. Consecuently, the number of root tips in the 2x seedlings was 2.9-fold greater than in the 4x plants (Figure ?Figure55). Table 5 Dry weight (g) of organs and whole plant of the 2x and 4x CC seedlings grown under greenhouse conditions (Experiment 2) for 10 weeks in nutrient solutions supplemented with 50 ��M (Ct) or 800 ��M (+B) H3BO3. FIGURE 5 Number of root tips in 6-month-old 2x and 4x CC seedlings grown in greenhouse conditions. Values are the means �� SE of three plants (n = 3). Different letters indicate significant differences at P