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The time of most recent typical ancestor (tMRCA) may differ from lineage to lineage (Table two)
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The total cellular ATP level was measured by an ATP bioluminescence detection assayThe intracellular ROS level was determined by a DCFH-DA fluorescence ROS detection assay
The total sub-G1 population is gated (pink box) and anything above roughly 103 should be positive for annexin V
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The transfected cells ended up instantly transferred into 10 ml of SDM-seventy nine supplemented with G418 and hygromycin
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The two cysteine residues of the 5 amino acid residue array at each of the two response centres
The two reaction centres belongs to one particular of 10 recognized types of plant proteinase inhibitors
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The uncoated upper side of each filter was wiped with a cotton swab to remove cells that had not migrated through the filter
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The upper benefit is in the variety of experimental values calculated with other systems
The upregulation of HSP60 and PDI by azacytidine shows that the endoplasmic reticulum and mitochondria are the major targets of azacytidine-induced cell stress
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The uptake was terminated by the removal of the substratecontaining buffer, and the wells were washed three times with icecold HBSS
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The values of in vivo resistance factors for strains picked on each and every compound ranged amongst the pursuing values
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